Products/Services Used | Details | Operation |
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Peptide Library Services> | … Peptide standards were purchased from GenScript Biotech. FcX (422301) was purchased from Biolegend, and anti-HLA-A, B, C (W6/32) … Institute for Molecular, G.; Cold Spring Harbor Laboratory, LAHGC; Biotechnology, GBGRC f.; Genome Analysis, G.; Scientific management: … | Get A Quote |
MHC-I peptides are a group of important immunopeptides presented by major histocompatibility complex (MHC) on the cell surface for immune recognition. The majority of reported MHC-I peptides are derived from protein coding sequences, and noncanonical peptides translated from small open reading frames (sORF) are largely unknown due to the lack of accurate and sensitive detection methods. Herein we report an efficient approach that implements complementary bioinformatic strategies to improve the identification of noncanonical MHC-I peptides. In a database search strategy, noncanonical immunopeptides mapping was optimized by combining three complementary pipelines to construct predicted sORF databases from Ribo-se... More